To add one or more filters, click "Filter" in the "Add" submenu on the left part of the screen. Note: if an excitation source is added to the graph, the rightmost column in the information table at the bottom of the page, (labeled "Peak Intensity" with the excitation source title in parentheses) will show the percentage of the maximum possible intensity for the emission curve of each compound currently on the graph. To exit out of the menu, click the small "x" on the upper right corner of the gray bar at the top of the menu. Once all desired options have been highlighted or selected, click the yellow "Add to graph" button on the lower right side. To search for an appropriate excitation source, use the right-side section to search by part type, manufacturer, or instrument. The excitation source will now appear on the graph display as a thin vertical line in a color associated with its location on the spectrum. Then repeat the process with the bandwidth number. To continue, enter the center wavelength value as a number and click "OK". If the "Custom" row is selected, a popup box will appear and prompt the user to enter the center wavelength. To change the current selection, click a different excitation source title. The choice currently selected will be highlighted in blue. If the desired excitation source is known, click to select. A menu will appear below the graph display with common generic lasers displayed on the left. To add one or more excitation sources, click "Excitation Source" in the "Add" submenu on the left part of the screen. To remove a compound, click the "x" to the right of the compound name. (Reminder: hold the SHIFT or CTRL key while clicking to select multiple dyes) Once all the desired options are highlighted blue, click the yellow "Add to graph" button on the lower right. The left-side menu will display all dyes with the selected properties. To search for dyes by feature, use the section on the right and enter in the desired values, then press the ENTER key. To select all of a range of dyes, click the first desired choice, hold the SHIFT key while clicking the dye at the end of the desired range. To select multiple individual options at the same time, hold the CTRL key while clicking each desired dye. To change the current selection, click a different dye name. To view a known dye, scroll down the menu on the left and click to select. To add one or more spectra to the graph, click either "fluorophore" in the "Add" submenu on the left or click the center of the empty display panel - a menu will appear at the bottom of the page.
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